The aseptic technique implies the practice and procedures to prevent contamination from a pathogen. The technique makes it possible to transfer bacterial culture into the fresh medium without introducing contaminants pathogens. Fresh media should be labeled with the corresponding name of the culture. Inoculating loop and inoculating needle are the main tools used in the aseptic techniques. Bunsen burner flame is used to sterilize inoculating tools. The tools are passed through the hottest part of the burner, which is blue. Avoid placing the sterilizing tools on the bench or touching it to prevent contamination.